Phosphonyl hydroxyacyl amino acid derivatives as antihypertensives

ABSTRACT

Phosphonyl hydroxyacyl amino acids of the formula &lt;IMAGE&gt;  wherein X is a substituted or unsubstituted imino or amino acid or ester. These compounds possess angiotensin converting enzyme activity and are thus useful as hypotensive agents.

BACKGROUND OF THE INVENTION

Thorsett, et al. in U.S. Pat. No. 4,316,896 disclose phosphoryl derivatives of aminoacids including proline. These compounds are disclosed as being hypotensive agents due to their angiotensin converting enzyme inhibition activity.

Petrillo in U.S. Pat. No. 4,168,267 discloses that various phosphinylalkanoyl substituted prolines are useful as hypotensive agents due to their ability to inhibit the angiotensin converting enzyme.

Ondetti et al. in U.S. Pat. No. 4,151,172 disclose that various phosphonoacyl prolines are useful as hypotensive agents due to their ability to inhibit the angiotensin converting enzyme.

Mercaptoacyl derivatives of proline and substituted prolines are known to be useful hypotensive agents due to their angiotensin converting enzyme inhibition activity. Ondetti et al. in U.S. Pat. No. 4,105,776 disclose such compounds wherein the proline ring is unsubstituted or substituted by an alkyl or hydroxy group. Ondetti et al. in U.S. Pat. No. 4,154,935 disclose such compounds wherein the proline ring is substituted with one or more halogens. Ondetti et al. in U.K. Patent Application No. 2,028,327 disclose such compounds wherein the proline ring is substituted by various ethers and thioethers. Krapcho in U.S. Pat. No. 4,217,359 discloses such compounds wherein the proline ring has a carbamoyloxy substituent. Krapcho in U.K. Patent Application No. 2,039,478 discloses compounds wherein the proline ring has a diether, dithioether, ketal or thioketal substituent in the 4-position. Krapcho in U.S. Pat. No. 4,316,905 discloses such compounds wherein the proline ring has a cycloalkyl, phenyl, or phenyl-lower alkylene substituent. Ondetti et al. in U.S. Pat. No. 4,234,489 disclose such compounds wherein the proline has a keto substituent in the 5-position. Krapcho et al. in U.S. Pat. No. 4,310,461 disclose such compounds wherein the proline has an imido, amido, or amino substituent in the 4-position. Iwao et al. in U.K. Patent Application No. 2,027,025 disclose such compounds wherein the proline has an aromatic substituent in the 5-position. Ondetti et al. in U.S. Pat. Nos. 4,053,651 and 4,199,512 disclose that mercaptoacyl derivatives of various aminoacids other than proline are also useful angiotensin converting enzyme inhibitors.

Karanewsky and Petrillo in U.S. Ser. No. 289,671 disclose phosphonamidate substituted amine or imino acids.

Mercaptoacyl derivatives of 3,4-dehydroproline are disclosed as angiotensin converting enzyme inhibitors by Ondetti in U.S. Pat. No. 4,129,566. Mercaptoacyl derivatives of thiazolidinecarboxylic acid and substituted thiazolidinecarboxylic acid are disclosed as angiotensin converting enzyme inhibitors by Ondetti in U.S. Pat. No. 4,192,878 and by Yoshitomo Pharmaceutical Ind. in Belgian Patent No. 868,532.

SUMMARY OF THE INVENTION

This invention is directed to new phosphonate substituted amino or imino acids of formula I and salts thereof ##STR2##

X is an imino or amino acid of the formula ##STR3##

R₄ is hydrogen, lower alkyl, halogen, keto, hydroxy, ##STR4## a 1- or 2-naphthyl of the formula ##STR5## a 1- or 2-naphthyloxy of the formula ##STR6## or a 1- or 2-naphthylthio of the formula ##STR7##

R₅ is keto, halogen, ##STR8## --O--lower alkyl, a 1- or 2-naphthyloxy of the formula ##STR9## --S--lower alkyl, ##STR10## or a 1- or 2-naphthylthio of the formula ##STR11##

R₇ is keto or ##STR12##

Each R₈ is independently halogen or --Y--R₁₄.

R₉, R₉ ', R₁₀ and R₁₀ ' are independently selected from hydrogen and lower alkyl or R₉ ', R₁₀ and R₁₀ ' are hydrogen and R₉ is ##STR13##

R₁₁ is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenylmethyl.

R₁₂ is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, or hydroxy.

m is zero, one, two or three.

p is one, two or three provided that p is more than one only if R₁₁ or R₁₂ is hydrogen, methyl, methoxy, chloro, or fluoro.

R₁₃ is hydrogen or lower alkyl of 1 to 4 carbons.

Y is oxygen or sulfur.

R₁₄ is lower alkyl of 1 to 4 carbons, ##STR14## or the R₁₄ groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons or a di(lower alkyl of 1 to 4 carbons) substituent.

R₂₁ is hydrogen, lower alkyl, cycloalkyl, phenyl, or ##STR15##

R₂₂ is hydrogen, lower alkyl, ##STR16##

r is an integer from 1 to 4.

R₁ is alkyl of 1 to 10 carbons, aminoalkyl, haloalkyl, ##STR17## wherein q is zero or an integer from 1 to 7 and R₁₂ and p are as defined above.

R₁₉ and R₂₀ are independently selected from hydrogen, lower alkyl, halo substituted lower alkyl, ##STR18## wherein m, R₁₁, and p are as defined above.

R₂ is hydrogen, lower alkyl, halo substituted lower alkyl, ##STR19## wherein r is as defined above.

R₃ and R₆ are independently selected from hydrogen, lower alkyl, benzyl, alkali metal such as Li, Na or K, benzhydryl, or ##STR20## wherein R₁₅ is hydrogen, lower alkyl, cycloalkyl, or phenyl, and R₁₆ is hydrogen, lower alkyl, lower alkoxy, phenyl, or R₁₅ and R₁₆ taken together are --(CH₂)₂ --, --(CH₂)₃ --, --CH═CH--, or ##STR21##

R₁₇ is lower alkyl, benzyl, or phenethyl.

R₁₈ is hydrogen, lower alkyl, benzyl or phenethyl.

DETAILED DESCRIPTION OF THE INVENTION

This invention in its broadest aspects relates to the phosphonate substituted imino or amino acid compounds of formula I above, to compositions containing such compounds and to the method of using such compounds as anti-hypertensive agents.

The term alkyl used in defining R₁ refers to straight or branched chain hydrocarbon radicals having up to ten carbons, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl, isopentyl, heptyl, octyl, decyl, etc. The term lower alkyl used in defining various symbols refers to straight or branched chain radicals having up to seven carbons. The preferred lower alkyl groups are up to four carbons with methyl and ethyl most preferred. Similarly the terms lower alkoxy and lower alkylthio refer to such lower alkyl groups attached to an oxygen or sulfur.

The term cycloalkyl refers to saturated rings of 3 to 7 carbon atoms with cyclopentyl and cyclohexyl being most preferred.

The term halo refers to Cl, Br and F. The term halo substituted lower alkyl refers to such lower alkyl groups described above in which one or more hydrogens have been replaced by chloro, bromo or fluoro groups such as trifluoromethyl, which is preferred, pentafluoroethyl, 2,2,2-trichloroethyl, chloromethyl, bromomethyl, etc. Similarly, the term amino substituted lower alkyl refers to lower alkyl groups in which one or more hydrogens have been replaced by --NH₂, i.e., aminomethyl, 2-aminoethyl, etc. The symbols ##STR22## represent that the alkylene bridge is attached to an available carbon atom.

The compounds of formula I wherein R₁ is other than ##STR23## are prepared according to the following procedures. A phosphonic acid of formula II ##STR24## wherein R₁ is as defined above is treated with a chlorinating agent such as phosphorus pentachloride in the presence of an inert organic solvent such as benzene to form a compound of the formula ##STR25## which is reacted with a lactate of the formula ##STR26## in the presence of an organic base such as triethylamine followed by an alcohol R₃ OH (where R₃ is lower alkyl, benzyl, or benzhydryl) to form a compound of the formula ##STR27## The formula V compound is then treated with strong base such as sodium hydroxide or lithium hydroxide in a mixture of water and an organic solvent such as dioxane to form the corresponding acid ##STR28##

The acid VI or its activated form is then coupled with an imino or amino acid or ester of the formula ##STR29## The term activated form refers to the conversion of the acid to a mixed anhydride, symmetrical anhydride, acid chloride, or activated ester, see Methoden der Organischen Chemie (Houben-Weyl), Vol. XV, part II, page 1 et seq. (1974) for a review of the methods of acylation. Preferably the reaction is performed in the presence of a coupling agent such as 1,1-carbonyldiimidazole, thionyl chloride, or dicyclohexylcarbodiimide.

In the above reaction if R₂ is ##STR30## then the hydroxyl, amino, imidazolyl, mercaptan, or guanidinyl function should be protected during the coupling reaction. Suitable protecting groups include benzyloxycarbonyl, t-butoxycarbonyl, benzyl, benzhydryl, trityl, etc., and nitro in the case of guanidinyl. The protecting group is removed by hydrogenation, treatment with acid, or other known methods following completion of the reaction.

Similarly, if in the above reaction R₁ =aminoalkyl, then the amino group should be similarly protected, preferably by phthalidyl. The protecting group is removed by treatment with hydrazine following completion of the reaction.

The products of formula I wherein either or both of R₃ and R₆ are lower alkyl, benzyl, or benzhydryl can be hydrogenated, for example, by treating with hydrogen in the presence of a palladium on carbon catalyst or chemically treated such as with sodium hydroxide in aqueous dioxane or with trimethylsilylbromide in dichloromethane to yield the products of formula I wherein R₃ and R₆ are hydrogen.

The ester products of formula I wherein R₆ is ##STR31## may be obtained by employing the imino or amino acid of formula V in the above reactions with the ester group already in place. Such ester reactants can be prepared by treating peptide, imino, or amino acids with an acid chloride such as ##STR32## so as to protect the N-atom. The protected acid compound is then reacted in the presence of base with a compound of the formula ##STR33## wherein L is a leaving group such as chlorine, bromine, tolylsulfonyloxy, etc., followed by removal of the N-protecting group such as by treatment with acid or hydrogenation.

The ester products of formula I wherein R₆ is ##STR34## can also be obtained by treating the product of formula I wherein R₆ is hydrogen with a molar equivalent of the compound of formula VIII. The diester products wherein R₃ and R₆ are the same and are ##STR35## can be obtained by treating the product of formula I wherein R₃ and R₆ are both hydrogen or an alkali metal salt with two or more equivalents of the compound of formula VIII.

The ester products of formula I wherein R₃ is ##STR36## can be obtained by treating the product of formula I wherein R₃ is hydrogen or an alkali metal salt and R₆ is benzyl or benzhydryl with the compound of formula VIII in the presence of base. Removal of the R₆ ester group such as by hydrogenation yields the products of formula I wherein R₃ is ##STR37## and R₆ is hydrogen.

The products of formula I wherein R₄ is amino may be obtained by reducing the corresponding products of formula I wherein R₄ is azido.

The various imino and amino acids and esters of formula V are described in the literature and in the various patents and pending U.S. application referred to above. Various substituted prolines are also disclosed by Mauger et al., Chem. Review, Vol. 66, p. 47-86 (1966). When the amino or imino acid is known, it can be readily converted to the ester by conventional means. For example, the esters where R₆ is t-butyl can be obtained by treating the corresponding N-carbobenzyloxyimino acid with isobutylene under acidic conditions and then removing the N-carbobenzyloxy protecting group by catalytic hydrogenation and the esters wherein R₆ is benzyl can be obtained by treating the imino acid with benzyl alcohol and thionyl chloride.

As disclosed by Krapcho in U.S. Ser. No. 164,985, the substituted prolines wherein R₄ is ##STR38## are prepared by reacting a 4-keto proline of the formula ##STR39## with a solution of the Grignard or lithium reagent ##STR40## wherein R₄ is as defined above and halo is Br or Cl to yield ##STR41## This compound is treated with a dehydrating agent such as p-toluenesulfonic acid, sulfuric acid, potassium bisulfate, or trifluoroacetic acid to yield the 3,4-dehydro-4-substituted proline of the formula ##STR42## Removal of the N-benzyloxycarbonyl protecting group and hydrogenation of the compound of formula XII yields the desired starting materials. The substituted proline wherein R₄ is cyclohexyl can be prepared by further hydrogenation of the 4-phenyl proline compound.

The substituted prolines wherein R₄ is the substituted amino group ##STR43## may be prepared by reacting a 4-keto proline of formula IX with the amine ##STR44## in the presence of hydrogen and catalyst or in the presence of sodium cyanotrihydridoborate.

The compounds of formula I wherein R₁ is ##STR45## that is ##STR46## may be prepared by reacting an aminophosphonic acid of the formula ##STR47## with an acid chloride having the formula ##STR48## such as benzoyl chloride, in the presence of an inert organic solvent, such as dioxane and a weak organic base, such as triethylamine to yield ##STR49## The formula XVI compound is then coupled with an imino or amino acid or ester of formula XVII ##STR50## in the presence of a coupling agent, such as dicyclohexylcarbodiimide as described above to form ##STR51##

Where X includes a protecting group, it may be removed by hydrogenation wherein the protecting group is phenylmethoxycarbonyl or by treatment with hydrazine where the protecting group is phthalidyl to yield the compounds of formula XIII.

The compounds of formula XVII may be prepared by coupling a hydroxy acid of formula XIX as the free acid or corresponding sodium salt ##STR52## with an imino or amino ester of formula VII preferably in the presence of a coupling agent such as diphenyl phosphorylazide.

Preferred compounds of this invention with respect to the amino or imino acid or ester part of the structure of formula I are those wherein:

R₂₁ is hydrogen, methyl, phenyl, cyclopentyl or cyclohexyl;

R₂₂ is hydrogen, lower alkyl of 1 to 4 carbons, ##STR53## R₆ is hydrogen, an alkali metal salt, or ##STR54## R₁₅ is hydrogen, methyl or isopropyl and R₁₆ is straight or branched chain lower alkyl of 1 to 4 carbons or phenyl.

R₄ is hydrogen.

R₄ is hydroxy.

R₄ is chloro or fluoro.

R₄ is lower alkyl of 1 to 4 carbons or cyclohexyl.

R₄ is amino.

R₄ is --O--lower alkyl wherein lower alkyl is straight or branched chain of 1 to 4 carbons.

R₄ is ##STR55## wherein m is zero, one or two, R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.

R₄ is ##STR56## 1-naphthyloxy, or 2-naphthyloxy wherein m is zero, one or two, and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.

R₄ is --S--lower alkyl wherein lower alkyl is straight or branched chain of 1 to 4 carbons.

R₄ is ##STR57## 1-naphthylthio, or 2-naphthylthio wherein m is zero, one or two, and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.

R₅ is --O--lower alkyl wherein lower alkyl is straight or branched chain of 1 to 4 carbons.

R₅ is ##STR58## wherein m is zero, one or two, and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.

R₅ is --S--lower alkyl wherein lower alkyl is straight or branched chain of 1 to 4 carbons.

R₅ is ##STR59## wherein m is zero, one or two, and R₁₂ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.

R₇ is phenyl, 2-hydroxyphenyl, or 4-hydroxyphenyl.

Each R₈ is independently fluoro or chloro.

Each R₈ is independently --Y--R₁₄ wherein Y is O or S, R₁₄ is straight or branched chain alkyl of 1 to 4 carbons or the R₁₄ groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a methyl or dimethyl substituent.

R₉, R₉ ', R₁₀ and R₁₀ ' are all hydrogen, or R₉ is phenyl, 2-hydroxyphenyl or 4-hydroxyphenyl and R₉ ', R₁₀ and R₁₀ ' are hydrogen.

Most preferred compounds of this invention with respect to the amino or imino acid or ester part of the structure of formula I are those wherein: ##STR60##

R₆ is hydrogen, ##STR61## or an alkali metal salt.

R₄ is hydrogen.

R₄ is cyclohexyl.

R₄ is lower alkoxy of 1 to 4 carbons.

R₄ is ##STR62## wherein m is zero, one, or two and R₁₁ is hydrogen, methyl, methoxy, methylthio, Cl, Br, F or hydroxy.

Y is oxygen or sulfur and t is two or three, especially wherein Y is sulfur and t is two.

Preferred compounds of this invention with respect to the phosphonyl sidechain of the structure of formula I are those wherein:

R₂ is hydrogen, lower alkyl of 1 to 4 carbons, CF₃, or amino substituted lower alkyl of 1 to 4 carbons, especially hydrogen, methyl or --(CH₂)₄ NH₂.

R₃ is hydrogen, an alkali metal salt, lower alkyl of 1 to 4 carbons, or ##STR63## wherein R₁₅ is hydrogen, methyl or isopropyl and R₁₆ is straight or branched chain lower alkyl of 1 to 4 carbons or phenyl, especially hydrogen, alkali metal salt, ethyl, ##STR64##

R₁ is alkyl of 1 to 10 carbons; ##STR65## wherein q is zero or an integer from 1 to 4 and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy; --(CH₂)_(q) -- cycloalkyl wherein cycloalkyl is of 5 or 6 carbons and q is zero, one or two; ##STR66## wherein q is zero or an integer from 1 to 4, ##STR67## wherein q is zero or an integer from 1 to 4, ##STR68## wherein q is zero or an integer from 1 to 4 or ##STR69##

R₁₉ and R₂₀ are independently selected from lower alkyl of 1 to 4 carbons or ##STR70## wherein q is zero or an integer from 1 to 4 and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy, especially wherein R₁₉ is phenylethyl and R₂₀ is phenyl.

The compounds of this invention wherein at least one of R₃ or R₆ is hydrogen, form basic salts with various inorganic and organic bases which are also within the scope of the invention. Such salts include ammonium salts, alkali metal salts like lithium, sodium and potassium salts (which are preferred), alkaline earth metal salts like calcium and magnesium salts, salts with organic bases, e.g., dicyclohexylamine salt, benzathine, N-methyl-D-glucamine, hydrabamine salts, salts with amino acids like arginine, lysine and the like. The nontoxic physiologically acceptable salts are preferred, although other salts are also useful, e.g., in isolating or purifying the product. The salts are formed using conventional techniques.

As shown above, the amino or imino acid or ester portion of the molecule of the products of formula I represented by X is in the L-configuration. Depending upon the definition of R₂ and R₁₉ other asymmetric center may be present in the phosphonyl sidechain. Thus, some of the compounds can accordingly exist in diastereoisomeric forms or in mixtures thereof. The above described processes can utilize racemates, enantiomers or diastereomers as starting materials. When diastereomeric products are prepared, they can be separated by conventional chromatographic or fractional crystallization methods.

The products of formula I wherein the imino acid ring is monosubstituted give rise to cis-trans isomerism. The configuration of the final product will depend upon the configuration of the R₄, R₅ and R₇ substituent in the starting material.

The compounds of formula I, and the physiologically acceptable salts thereof, are hypotensive agents. They inhibit the conversion of the decapeptide angiotensin I to angiotensin II and, therefore, are useful in reducing or relieving angiotensin related hypertension. The action of the enzyme renin on angiotensinogen, a pseudoglobulin in blood pressure, produces angiotensin I. Angiotensin I is converted by angiotensin converting enzyme (ACE) to angiotensin II. The latter is an active pressor substance which has been implicated as the causative agent in several forms of hypertension in various mammalian species, e.g., humans. The compounds of this invention intervene in the angiotensinogen→(renin)→angiotensin I→angiotensin II sequence by inhibiting angiotensin converting enzyme and reducing or eliminating the formation of the pressor substance angiotensin II. Thus by the administration of a composition containing one (or a combination) of the compounds of this invention, angiotensin dependent hypertension in a species of mammal (e.g., humans) suffering therefrom is alleviated. A single dose, or preferably two or four divided daily doses, provided on a basis of about 0.1 to 100 mg per kilogram of body weight per day is appropriate to reduce blood pressure. The substance is preferably administered orally, but parenteral routes such as the subcutaneous, intramuscular, intravenous or intraperitoneal routes can also be employed.

The compounds of this invention can also be formulated in combination with a diuretic for the treatment of hypertension. A combination product comprising a compound of this invention and a diuretic can be administered in an effective amount which comprises a total daily dosage of about 30 to 600 mg., preferably about 30 to 330 mg of a compound of this invention, and about 15 to 300 mg, preferably about 15 to 200 mg of the diuretic, to a mammalian species in need thereof. Exemplary of the diuretics contemplated for use in combination with a compound of this invention are the thiazide diuretics, e.g., chlorothiazide, hydrochlorothiazide, flumethiazide, hydroflumethiazide, bendroflumethiazide, methylclothiazide, trichloromethiazide, polythiazide or benzthiazide as well as ethacrynic acid, ticrynafen, chlorthalidone, furosemide, musolimine, bumetanide, triamterene, amiloride and spironolactone and salts of such compounds.

The compounds of formula I can be formulated for use in the reduction of blood pressure in compositions such as tablets, capsules or elixirs for oral administration, or in sterile solutions or suspensions for parenteral administration. About 10 to 500 mg of a compound of formula I is compounded with physiologically acceptable vehicle, carrier, excipient, binder, preservatives, stabilizer, flavor, etc., in a unit dosage form as called for by accepted pharmaceutical practice. The amount of active substance in these compositions or preparations is such that a suitable dosage in the range indicated is obtained.

The following examples are illustrative and present preferred embodiments of the invention. Temperatures are given in degrees centigrade. AG-50W-X8 refers to a crosslinked polystyrenedivinylbenzene sulfonic acid cation exchange resin. HP-20 refers to a porous crosslinked polystyrene-divinyl benzene polymer resin.

EXAMPLE 1 (±)-1-[2-[[Hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, dilithium salt A. (4-Phenylbutyl)phosphonic acid

A mixture of 4-phenylbutyl chloride (8.0 g, 47.5 mmole) and triethylphosphite (15.0 ml, 72 mmole) was heated at reflux (bath temperature 185° C.) under argon for 41.5 hours. Distillation of the mixture gave pure diethyl (4-phenylbutyl)phosphonate (10.8 g, 84%) as a colorless liquid, b.p. 152°-154° C. (1.0 mmHg). TLC (EtOAc) single spot R_(f) =0.55.

A mixture of diethyl (4-phenylbutyl)phosphonate (3.5 g, 13.0 mmole) and 6 N HCl (45 ml) was refluxed under argon for 16 hours. The cooled reaction mixture was extracted with EtOAc. The organic phase was washed with saturated NaCl, dried (MgSO₄), and evaporated. The crude product (2.3 g) was recrystallized from diisopropyl ether to give pure (4-phenylbutyl)phosphonic acid (1.7 g, 61%) as white needles, m.p. 92°-93° C.

Analysis Calcd for C₁₀ H₁₅ O₃ P: C, 56.07; H, 7.06; P, 14.46. Found: C, 55.83; H, 7.04; P. 14.34.

B. (±)-2-[[Phenylmethoxy(4-phenylbutyl)phosphinyl]oxy]propionic acid, ethyl ester

A mixture of 4-phenylbutyl phosphonic acid from Part A (0.70 g, 3.27 mmole), benzene (10 ml), and PCl₅ (1.36 g, 6.54 mmole) was refluxed under argon for 30 minutes. The benzene and POCl₃ were removed in vacuo and the residue taken up in CH₂ Cl₂ (5 ml). After cooling to 0° C. (ice-bath), triethylamine (1.3 ml, 9.39 mmole) was added followed by dropwise treatment with d,1 ethyl lactate (0.39 ml, 3.3 mmole) in CH₂ Cl₂ (3 ml) over a 5 minute period. After 1 hour benzyl alcohol (0.35 ml, 3.3 mmole) in CH₂ Cl₂ (3 ml) was added dropwise over 2 minutes, the ice-bath removed, and the reaction mixture allowed to stir for 2 hours. The reaction mixture was diluted with EtOAc then washed with H₂ O, 5% KHSO₄, saturated NaHCO₃, brine, dried (MgSO.sub. 4), and evaporated. The residue (1.3 g) was chromatographed on silica (70 g) eluting with 2/1 hexane/EtOAc to obtain the title compound (0.80 g, 1.98 mmole; 60% yield) as an oil.

TLC (2/1 hexane/EtOAc) two isomers R_(f) =0.25, 0.20.

C. (±)-2-[[Phenylmethoxy(4-phenylbutyl)phosphinyl]oxy]propionic acid

A mixture of the ethyl ester from Part B (0.80 g, 1.98 mmole), 1 N NaOH (3.0 ml, 3.0 mmole) and dioxane (10 ml) was stirred at 25° C. in an argon atmosphere for 2 hours. The reaction mixture was diluted with H₂ O and then washed with EtOAc. The aqueous phase was acidified to pH=1.0 with concentrated HCl and the resulting oil was extracted into EtOAc. The ETOAc extract was washed with brine, dried (MgSO₄), and evaporated to give the title compound (0.70 g, 1.86 mmole, 94% yield) as an oil.

TLC (1/9 CH₃ OH/CH₂ Cl₂) major spot R_(f) =0.5.

D. (±)-1-[2-[[Phenylmethoxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, phenylmethyl ester

A mixture of mono acid from Part C (0.70 g, 1.86 mmole), 1,1-carbonyldiimidazole (0.30 g, 1.85 mmole) and dry THF (10 ml) was stirred at 0° C. in an argon atmosphere for 1 hour. Triethylamine (0.26 ml, 1.88 mmole) and L-proline, phenylmethyl ester, hydrochloride salt (commercially available) (0.45 g, 1.86 mmole) were added to the resulting imidazolide and the ice-bath removed. After 60 hours the reaction mixture was diluted with EtOAc, then washed with H₂ O, 5% KHSO₄, saturated NaHCO₃, brine, dried (MgSO₄), and evaporated. The residue (850 mg) was chromatographed on silica (45 g) eluting with 5/2 hexane/acetone to obtain the title compound (0.40 g, 0.71 mmole, 38% yield) as an oil.

TLC (5/2 hexane/acetone) single spot R_(f) =0.2.

E. (±)-1-[2-[[Hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, dilithium salt

A mixture of the dibenzyl ester from Part D (0.40 g, 0.71 mmole), 10% Pd/C (50 mg), and CH₃ OH (40 ml) was hydrogenated on the Parr apparatus at 50 psi for 3 hours. The catalyst was removed by filtration (celite bed) and the filtrate was evaporated. The residue was taken up in H₂ O (2 ml) and 0.1 M Li₂ CO₃ (3.5 ml, 0.35 mmole) and passed through an AG50WX8 (Li) (40 ml) column. The desired fractions were combined, filtered (millipore), and lyophilized to give the title product (245 mg, 0.62 mmole, 87% yield) as a glassy solid.

TLC (7/2/1 isopropanol/conc. NH₄ OH/H₂ O) single spot R_(f) =0.8.

Analysis calcd for C₁₈ H₂₄ NO₆ P.2 Li.1.0 mole H₂ O: C, 52.31; H, 6.34; N, 3.39; P, 7.5. Found: C, 52.44; H, 6.14; N, 3.63; P, 7.2.

EXAMPLE 2 1-[(S)-2-[[[(±)-1-(Benzoylamino)-3-phenylpropyl]phosphinyl]oxy]-1-oxopropyl]-L-proline, dilithium salt A. (1-Amino-3-phenylpropyl)phosphonic acid

To a stirred solution of benzyl carbamate (15 g, 0.1 mole) and phosphorus trichloride (9 ml, 0.1 mole) in glacial acetic acid (25 ml) at 0° (ice bath), there is added 3-phenylpropanol (20 g, 0.149 mole) dropwise over a period of 30 minutes. The resulting mixture is stirred at 0° for 15 minutes and then allowed to warm to room temperature. The mixture is then refluxed for 30 minutes, treated with 4 N hydrochloric acid (125 ml) and again refluxed for one hour. After cooling, the aqueous solution is decanted from the dark organic layer, washed with ethyl acetate, and evaporated to dryness. The residue is taken up in water (50 ml) and again evaporated to dryness. This is repeated two more times. Finally, the solid residue is triturated with acetonitrile-water and dried in vacuo over phosphorus pentoxide to give 10.05 g of (1-amino-3-phenylpropyl)phosphonic acid as a white crystalline solid; m.p. 274°-278° (dec.).

B. [3-Phenyl-1-[(benzoylamino)propyl]phosphonic acid

A mixture of amino phosphonic acid prepared in Part A (3.2 g, 14.9 mmole), dioxane (20 ml), H₂ O (8.0 ml), and triethylamine (7.5 ml, 54.2 mmole) at 0° C. (ice bath) was treated dropwise with benzoyl chloride (2.8 ml, 19.4 mmole) in dioxane (4.0 ml) over a 5 minute period. The ice bath was removed and the reaction mixture stirred for 2 hours, diluted with H₂ O, and then washed with Et₂ O. The aqueous phase was acidified to pH 1.0 with concentrated HCl and the resulting oil was extracted into EtOAc (3x), washed with brine, dried (MgSO₄), and evaporated. The residue was stirred under Et₂ O/hexane to give a gummy solid, from which upon trituration with IPE (2x) the title compound (4.0 g, 12.5 mmole, 84% yield) was obtained as a white crystalline solid, m.p. 166°-168° C.

TLC (7:2:1, Isopropanol/conc. NH₄ OH/H₂ O) major spot, R_(f) =0.4.

Analysis Calcd for C₁₀ H₁₃ NO₄ P: N, 4.39; C. 60.10; H, 5.68; P, 9.7. Found: N, 4.34; C, 60.30; H, 5.83; P, 9.6.

C. 1-[(S)-2-Hydroxy-1-oxopropyl]-L-proline, phenylmethyl ester

A mixture of sodium lactate (1.7 g, 15.0 mmole), diphenyl phosphorylazide (3.6 ml, 16.5 mmole) and dry DMF (30 ml) at 0° C. (ice bath) in an argon atmosphere was treated with triethyl amine (2.1 ml, 15.2 mmole) and L-proline, phenylmethyl ester, hydrochloride salt (3.6 g, 15.0 mmole). After 24 hours, the reaction mixture was partitioned between EtOAc and H₂ O. The aqueous phase was back extracted, the organic extracts combined, washed with 5% KHSO₄, brine, and evaporated. The residue (5.0 g) was chromatographed on silica (130 g) eluting the EtOAc/Hexane (1:1) to give the title compound (2.5 g, 9.0 mmole, 60% yield) as a white crystalline solid after evaporation, m.p. 86°-88° C.

Analysis Calcd for C₁₅ H₁₉ NO₄ : N, 5.05; C, 64.97; H, 6.91. Found: N, 5.02; C, 64.70; H, 6.85.

D. 1-[(S)-2-[[[(±)-1-(Benzoylamino)-3-phenylpropyl]hydroxyphosphinyl]oxy]-1-oxopropyl-L-proline, phenylmethyl ester

A mixture of the phosphonic acid from Part B (0.60 g, 1.9 mmole), lactoyl proline, phenylmethyl ester (from Part C) (0.52 g, 1.9 mmole), and dry THF (5 ml) at 0° C. under argon was treated with dicyclohexylcarbodiimide (0.39 g, 1.9 mmole). After 15 minutes, the reaction mixture was diluted with EtOAc and filtered to remove the dicyclohexylurea. The filtrate was washed with 5% KHSO₄, brine, dried (MgSO₄), and evaporated. The residue (1.2 g) was chromatographed on silica (60 g) eluting with (20:1:1, CH₂ Cl₂ /CH₃ OH/HOAc). The desired fractions were combined and evaporated to dryness to give the title compound (1.0 g, 1.7 mmole, 90% yield) as a foam. TLC: (20:1:1, CH₂ Cl₂ /CH₃ OH/HOAc) two isomers R_(f) =0.15, 0.20.

E. 1-[(S)-2-[[[(±)-1-(Benzoylamino)-3-phenylpropyl]phosphinyl]oxy]-1-oxopropyl]-L-proline, dilithium salt

A mixture of the phosphonic monoester from Part D (1.0 g, 1.7 mmole), 10% Pd/C (400 mg), and CH₃ OH (50 ml) was hydrogenated on the Parr apparatus at 50 psi for 1.5 hours. The catalyst was removed by filtration (Celite bed) and the solvent evaporated. The residue was taken up in 1 N LiOH (2.5 ml, 2.5 mmole) and applied to an AG50Wx8 (Li) (50 ml) column eluting with H₂ O. The desired fractions were combined, evaporated to small volume, and chromatographed on an HP-20 (200 ml) column eluting with a linear gradient H₂ O/CH₃ CN (0→90% CH₃ CN). The desired fractions were combined, evaporated to dryness, taken up in H₂ O, filtered (millipore), and lyophilized to give the title product (0.51 g, 1.0 mmole, 60% yield) as a white solid. TLC: (7:2:1, Isopropanol/conc. NH₂ OH/H₂ O) single spot R_(f) =0.7, m.p. 248°-255° C.

Analysis Calcd for C₂₄ H₂₇ N₂ O₇ PLi₂.0.74 moles of H₂ O

Calcd: N, 5.45; C, 56.12; H, 5.59; P, 6.0. Found: N, 5.35; C, 56.12; H, 5.66; P, 6.0.

EXAMPLES 3-105

Following the procedure of Example 1 but employing the phosphonic acid shown in Col. I, the lactate shown in Column II, the imino acid or amino acid or ester in Col. III, and the alcohol R₃ OH, one obtains the diester shown in Col. IV. Both the R₃ and R₆ ester groups may be removed to yield the corresponding diacid or salt as set forth in Example 1E or only the carboxylic ester group R₆ may be removed or in the case of Examples 77-87 only the R₃ ester group may be removed.

    ______________________________________                                         Col. I          Col. III                                                       ______________________________________                                          ##STR71##                                                                                      ##STR72##                                                     ______________________________________                                         Col. III        Col. IV                                                        ______________________________________                                         HX                                                                                              ##STR73##                                                     ______________________________________                                    

    __________________________________________________________________________     Ex.                                                                               R.sub.1        R.sub.3  R.sub.2        X                                    __________________________________________________________________________     3. H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR74##                                                                               ##STR75##                                                                                     ##STR76##                           4. H.sub.3 C                                                                                      ##STR77##                                                                              CH.sub.3                                                                                       ##STR78##                           5. H.sub.5 C.sub.2                                                                                ##STR79##                                                                              H                                                                                              ##STR80##                               ##STR81##                                                                                     ##STR82##                                                                              CH.sub.3                                                                                       ##STR83##                               ##STR84##                                                                                     ##STR85##                                                                               ##STR86##                                                                                     ##STR87##                               ##STR88##                                           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                                                 CH.sub.3                                                                                       ##STR119##                              ##STR120##                                                                                    ##STR121##                                                                             CF.sub.3                                                                                       ##STR122##                              ##STR123##                                                                                    ##STR124##                                                                             CH.sub.3                                                                                       ##STR125##                          20.                                                                                ##STR126##                                                                                    ##STR127##                                                                             CH.sub.3                                                                                       ##STR128##                              ##STR129##                                                                                    ##STR130##                                                                             CH.sub.3                                                                                       ##STR131##                             H.sub.3 C(CH.sub.2).sub.6                                                                      ##STR132##                                                                             CH.sub.3                                                                                       ##STR133##                             H.sub.3 C(CH.sub.2).sub.3                                                                      ##STR134##                                                                             CH.sub.3                                                                                       ##STR135##                              ##STR136##                                                                                    ##STR137##                                                                             CH.sub.3                                                                                       ##STR138##                              ##STR139##                                                                                    ##STR140##                                                                             H                                                                                              ##STR141##                              ##STR142##                                                                                    ##STR143##                                                                             CH.sub.3                                                                                       ##STR144##                              ##STR145##                                                                                    ##STR146##                                                                             CH.sub.3                                                                                       ##STR147##                              ##STR148##                                                                                    ##STR149##                                                                             CH.sub.3                                                                                       ##STR150##                              ##STR151##                                                                                    ##STR152##                                                                             CH.sub.3                                                                                       ##STR153##                          30.                                                                                ##STR154##                                                                                    ##STR155##                                                                             CH.sub.3                                                                                       ##STR156##                              ##STR157##                                                                                    ##STR158##                                                                             CH.sub.3                                                                                       ##STR159##                              ##STR160##                                                                                    ##STR161##                                                                             CH.sub.3                                                                                       ##STR162##                              ##STR163##                                                                                    ##STR164##                                                                             CH.sub.3                                                                                       ##STR165##                              ##STR166##                                                                                    ##STR167##                                                                             CH.sub.3                                                                                       ##STR168##                              ##STR169##                                                                                    ##STR170##                                                                             CH.sub.3                                                                                       ##STR171##                             H.sub.3 C                                                                                      ##STR172##                                                                             CH.sub.3                                                                                       ##STR173##                             H.sub.5 C.sub.2                                                                                ##STR174##                                                                             CH.sub.3                                                                                       ##STR175##                              ##STR176##                                                                                    ##STR177##                                                                             CH.sub.3                                                                                       ##STR178##                              ##STR179##                                                                                    ##STR180##                                                                             CH.sub.3                                                                                       ##STR181##                          40.                                                                                ##STR182##                                                                                    ##STR183##                                                                             CH.sub.3                                                                                       ##STR184##                          -41.                                                                               ##STR185##                                                                                    ##STR186##                                                                             CH.sub.3                                                                                       ##STR187##                             H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR188##                                                                             H                                                                                              ##STR189##                              ##STR190##                                                                                    ##STR191##                                                                             CH.sub.3                                                                                       ##STR192##                              ##STR193##                                                                                    ##STR194##                                                                              ##STR195##                                                                                    ##STR196##                              ##STR197##                                                                                    ##STR198##                                                                             CH.sub.3                                                                                       ##STR199##                              ##STR200##                                                                                    ##STR201##                                                                             CH.sub.3                                                                                       ##STR202##                              ##STR203##                                                                                    ##STR204##                                                                             CH.sub.3                                                                                       ##STR205##                              ##STR206##                                                                                    ##STR207##                                                                             CH.sub.3                                                                                       ##STR208##                             H.sub.3 C(CH.sub.2).sub.3                                                                      ##STR209##                                                                             CH.sub.3                                                                                       ##STR210##                          50.                                                                                ##STR211##                                                                                    ##STR212##                                                                             CH.sub.3                                                                                       ##STR213##                              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                                         ##STR224##                                                                             CH.sub.3                                                                                       ##STR225##                              ##STR226##                                                                                    ##STR227##                                                                             CH.sub.3                                                                                       ##STR228##                             H.sub.3 C(CH.sub.2).sub.3                                                                      ##STR229##                                                                             CH.sub.3                                                                                       ##STR230##                              ##STR231##                                                                                    ##STR232##                                                                             CH.sub.3                                                                                       ##STR233##                              ##STR234##                                                                                    ##STR235##                                                                             CH.sub.3                                                                                       ##STR236##                             H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR237##                                                                             CH.sub.3                                                                                       ##STR238##                          60.                                                                                ##STR239##                                                                                    ##STR240##                                                                             CH.sub.3                                                                                       ##STR241##                              ##STR242##                                                                                    ##STR243##                                                                             CH.sub.3                                                                                       ##STR244##                              ##STR245##                                                                                    ##STR246##                                                                             H                                                                                              ##STR247##                              ##STR248##                                                                                    ##STR249##                                                                             CH.sub.3                                                                                       ##STR250##                              ##STR251##                                                                                    ##STR252##                                                                             CH.sub.3                                                                                       ##STR253##                             H.sub.3 C(CH.sub.2).sub.4                                                                      ##STR254##                                                                             CH.sub.3                                                                                       ##STR255##                              ##STR256##                                                                                    ##STR257##                                                                             H                                                                                              ##STR258##                              ##STR259##                                                                                    ##STR260##                                                                             CH.sub.3                                                                                       ##STR261##                              ##STR262##                                                                                    ##STR263##                                                                             C.sub.2 H.sub.5                                                                                ##STR264##                              ##STR265##                                                                                    ##STR266##                                                                             CH.sub.3                                                                                       ##STR267##                          70.                                                                               H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR268##                                                                             CH.sub.3                                                                                       ##STR269##                              ##STR270##                                                                                    ##STR271##                                                                             CH.sub.3                                                                                       ##STR272##                              ##STR273##                                                                                    ##STR274##                                                                             CH.sub.3                                                                                       ##STR275##                              ##STR276##                                                                                    ##STR277##                                                                             CH.sub.3                                                                                       ##STR278##                              ##STR279##                                                                                    ##STR280##                                                                             CH.sub.3                                                                                       ##STR281##                              ##STR282##                                                                                    ##STR283##                                                                             CH.sub.3                                                                                       ##STR284##                             H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR285##                                                                             H                                                                                              ##STR286##                              ##STR287##                                                                                    ##STR288##                                                                             CH.sub.3                                                                                       ##STR289##                              ##STR290##                                                                                    ##STR291##                                                                             CH.sub.3                                                                                       ##STR292##                             H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR293##                                                                             CH.sub.3                                                                                       ##STR294##                          80.                                                                                ##STR295##                                                                                    ##STR296##                                                                             CH.sub.3                                                                                       ##STR297##                              ##STR298##                                                                                    ##STR299##                                                                             CH.sub.3                                                                                       ##STR300##                              ##STR301##                                                                                    ##STR302##                                                                             CH.sub.3                                                                                       ##STR303##                              ##STR304##                                                                                    ##STR305##                                                                             CH.sub.3                                                                                       ##STR306##                              ##STR307##                                                                                    ##STR308##                                                                             CH.sub.3                                                                                       ##STR309##                              ##STR310##                                                                                    ##STR311##                                                                             CH.sub.3                                                                                       ##STR312##                              ##STR313##                                                                                    ##STR314##                                                                             CH.sub.3                                                                                       ##STR315##                              ##STR316##                                                                                    ##STR317##                                                                             CH.sub.3                                                                                       ##STR318##                              ##STR319##                                                                                    ##STR320##                                                                             CH.sub.3                                                                                       ##STR321##                              ##STR322##                                                                                    ##STR323##                                                                             CH.sub.3                                                                                       ##STR324##                          90.                                                                               H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR325##                                                                             H                                                                                              ##STR326##                              ##STR327##                                                                                    ##STR328##                                                                             CH.sub.3                                                                                       ##STR329##                              ##STR330##                                                                                    ##STR331##                                                                             CH.sub.3                                                                                       ##STR332##                              ##STR333##                                                                                    ##STR334##                                                                             CH.sub.3                                                                                       ##STR335##                             H.sub.5 C.sub.2                                                                                ##STR336##                                                                             CH.sub.3                                                                                       ##STR337##                              ##STR338##                                                                                    ##STR339##                                                                             CH.sub.3                                                                                       ##STR340##                              ##STR341##                                                                                    ##STR342##                                                                             CH.sub.3                                                                                       ##STR343##                              ##STR344##                                                                                    ##STR345##                                                                             CH.sub.3                                                                                       ##STR346##                              ##STR347##                                                                                    ##STR348##                                                                             CH.sub.3                                                                                       ##STR349##                              ##STR350##                                                                                    ##STR351##                                                                             CH.sub.3                                                                                       ##STR352##                          100.                                                                               ##STR353##                                                                                    ##STR354##                                                                             CH.sub.3                                                                                       ##STR355##                          101.                                                                              H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR356##                                                                             CH.sub.3                                                                                       ##STR357##                          102.                                                                               ##STR358##                                                                                    ##STR359##                                                                             H                                                                                              ##STR360##                          103.                                                                              H.sub.3 C(CH.sub.2).sub.5                                                                      ##STR361##                                                                             CH.sub.3                                                                                       ##STR362##                          104.                                                                              H.sub.3 CCH.sub.2                                                                              ##STR363##                                                                             C.sub.2 H.sub.5                                                                                ##STR364##                          105.                                                                               ##STR365##                                                                                    ##STR366##                                                                             H                                                                                              ##STR367##                          __________________________________________________________________________

EXAMPLES 106 TO 124

Following the procedure of Example 2 but employing the phosphonic acid shown in Col. I, the acid chloride shown in Col. II, and the hydroxyacyl imino ester shown in Col. III, one obtains the intermediate shown in Col. IV. Removal of the carboxylic acid protecting group yields the compound of Col. V which can be treated to obtain a salt as shown in Example 2, Part E.

    ______________________________________                                         Col. I        Col. II     Col. III                                             ______________________________________                                          ##STR368##                                                                                   ##STR369##                                                                                 ##STR370##                                          ______________________________________                                         Col. IV                                                                        ______________________________________                                          ##STR371##                                                                    ______________________________________                                         Col. V                                                                         ______________________________________                                          ##STR372##                                                                    ______________________________________                                    

    __________________________________________________________________________     Ex.                                                                               R.sub.19   R.sub.2         X                   R.sub.20                     __________________________________________________________________________     106.                                                                               ##STR373##                                                                               CH.sub.3                                                                                        ##STR374##                                                                                         ##STR375##                  107.                                                                               ##STR376##                                                                               CH.sub.3                                                                                        ##STR377##                                                                                         ##STR378##                  108.                                                                               ##STR379##                                                                               CH.sub.3                                                                                        ##STR380##         H.sub.3 C                    109.                                                                               ##STR381##                                                                               CH.sub.3                                                                                        ##STR382##                                                                                         ##STR383##                  110.                                                                               ##STR384##                                                                               CH.sub.3                                                                                        ##STR385##                                                                                         ##STR386##                  111.                                                                               ##STR387##                                                                               CH.sub.3                                                                                        ##STR388##         F.sub.3 C                    112.                                                                               ##STR389##                                                                               CH.sub.3                                                                                        ##STR390##         H                            113.                                                                              CH.sub.3   CH.sub.3                                                                                        ##STR391##                                                                                         ##STR392##                  114.                                                                               ##STR393##                                                                               CH.sub.3                                                                                        ##STR394##                                                                                         ##STR395##                  115.                                                                               ##STR396##                                                                               CH.sub.3                                                                                        ##STR397##                                                                                         ##STR398##                  116.                                                                               ##STR399##                                                                               CH.sub.3                                                                                        ##STR400##                                                                                         ##STR401##                  117.                                                                               ##STR402##                                                                               CH.sub.3                                                                                        ##STR403##                                                                                         ##STR404##                  118.                                                                              H          CH.sub.3                                                                                        ##STR405##                                                                                         ##STR406##                  119.                                                                               ##STR407##                                                                                ##STR408##                                                                                     ##STR409##                                                                                         ##STR410##                  120.                                                                               ##STR411##                                                                               CH.sub.3                                                                                        ##STR412##                                                                                         ##STR413##                  121.                                                                               ##STR414##                                                                               H                                                                                               ##STR415##                                                                                         ##STR416##                  122.                                                                               ##STR417##                                                                               H                                                                                               ##STR418##                                                                                         ##STR419##                  123.                                                                               ##STR420##                                                                               C.sub.2 H.sub.5                                                                                 ##STR421##                                                                                         ##STR422##                  124.                                                                               ##STR423##                                                                               CH.sub.3                                                                                        ##STR424##                                                                                         ##STR425##                  __________________________________________________________________________

EXAMPLE 125 (±)-1-[2-[[[(2,2-Dimethyl-1-oxopropoxy)methoxy](4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline A. (±)-1-[2-[[Phenylmethoxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, 1,1-dimethylethyl ester

Following the procedure of Example 1, Part D, using L-proline, 1,1-dimethylethyl ester in place of L-proline, phenylmethyl ester gives the title compound.

B. (±)-1-[2-[[Hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, 1,1-dimethylethyl ester

A mixture of the benzyl ester from Part A (1.03 g, 2.0 mmole) 10% Pd/C (0.20 g) and methanol (50 ml) is hydrogenated on a Parr apparatus at a pressure of 50 psi for 3 hours. The catalyst is removed by filtration through celite and the filtrate evaporated to dryness to give the title compound.

C. (±)-1-[2-[[[(2,2-Dimethyl-1-oxopropyl)methoxy](4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, 1,1-dimethylethyl ester

A solution of the monoacid from Part B (0.64 g, 1.5 mmole), triethylamine (0.42 ml, 3.0 mmole) and chloromethyl pivalate (0.45 g, 3.0 mmole) in dry dimethylformamide (5 ml) is stirred at room temperature under argon for 16 hours. The mixture is then partitioned between EtOAc-water. The organic phase is washed successively with 5% KHSO₄, saturated NaHCO₃ and saturated NaCl, dried over Na₂ SO₄ and evaporated. The crude product is purified by flash chromatography on silica gel to give the title compound.

D. (±)-1-[2-[[[(2,2-Dimethyl-1-oxopropoxy)methoxy](4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline

A solution of the diester from Part C (0.54 g, 1.0 mmole) and anisole (2 ml) in CH₂ Cl₂ (10 ml) is treated with trifluoroacetic acid (5 ml) at 0° C. (ice bath). After 1 hour at 0° C., the mixture is partitioned between EtOAc-water. The organic phase is washed with water and saturated NaCl, dried over Na₂ SO₄ and evaporated. The crude product is purified by flash chromatography on silica gel to give the title compound.

EXAMPLES 126-130

Following the procedure of Example 125 but employing the alkylating agent shown in Col. I in place of the chloromethyl pivalate, one obtains the product in Col. II.

    ______________________________________                                         Ex.  Col. I           Col. II                                                  ______________________________________                                         126.                                                                                 ##STR426##      (±)-1-[2-[[[(Acetyloxy)- methoxy](4-phenylbutyl)-                            phosphinyl]oxy]-1-oxopropyl]- L-proline                 127.                                                                                 ##STR427##      (±)-1-[2-[[[1-(Ethoxycarbonyl- oxy)ethoxy](4-phen                           ylbutyl)- phosphinyl]oxy]-1-oxopropyl]- L-proline        128.                                                                                 ##STR428##      (±)-1-[2-[[(3-Oxo-1-iso- benzofuranyloxy)(4-pheny                           l- butyl)phosphinyl]oxy]-1- oxopropyl]-L-proline         129.                                                                                 ##STR429##      (±)-1-[2-[[[(Benzoyloxy)- methoxy](4-phenylbutyl)                           - phosphinyl]oxy]-1-oxopropyl]- L-proline                130.                                                                                 ##STR430##      (±)-1-[2-[[[2-Methyl-1- (1-oxopropoxy)propoxy](4-                            phenylbutyl)phosphinyl]oxy]- 1-oxopropyl]-L-proline     ______________________________________                                    

Similarly, the alkylating agents of Examples 125-130 can be employed with the appropriately protected compounds of Examples 1 to 124 to yield other compounds within the scope of this invention. In the cases where the proline carboxyl group is protected as its phenylmethyl ester rather than its t-butyl ester, it is removed by hydrogenation in the presence of Pd/C in the final step.

EXAMPLE 131 (±)-[2-[[Hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, disodium salt

Following the procedure of Example 1 but substituting AG-50W-X8 (Na⁺) for the lithium resin in Part E, one obtains the title product.

This procedure can be employed in Examples 2-130 to give the corresponding mono or disodium salt. Similarly, by employing a potassium resin the corresponding mono or dipotassium salt is obtained.

EXAMPLE 132

1000 Tablets each containing the following ingredients:

    ______________________________________                                         (±)-[2-[[Hydroxy(4-phenylbutyl)-                                                                     100    mg                                             phosphinyl]oxy]-1-oxopropyl]-                                                  L-proline, disodium salt                                                       Corn starch              50     mg                                             Gelatin                  7.5    mg                                             Avicel (microcrystalline 25     mg                                             cellulose)                                                                     Magnesium stearate       2.5    mg                                                                      185    mg                                             ______________________________________                                    

are prepared from sufficient bulk quantities by mixing the (±)-[2-[[hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, disodium salt and corn starch with an aqueous solution of the gelatin. The mixture is dried and ground to a fine powder. The Avicel and then the magnesium stearate are admixed with granulation. This mixture is then compressed in a tablet to form 1000 tablets each containing 100 mg of active ingredient.

In a similar manner, tablets containing 100 mg of the product of any of Examples 2 to 130 can be prepared.

EXAMPLE 133

1000 tablets each containing the following ingredients:

    ______________________________________                                         1-[(S)--2-[[[(±)-1-(Benzoylamino)-                                                                   50     mg                                             3-phenylpropyl]phosphinyl]oxy]-                                                1-oxopropyl]-L-proline, sodium salt                                            Lactose                  25     mg                                             Avicel                   38     mg                                             Corn starch              15     mg                                             Magnesium stearate       2      mg                                                                      130    mg                                             ______________________________________                                    

are prepared from sufficient bulk quantities by mixing the 1-[(S)-2-[[[(±)-1-(benzoylamino)-3-phenylpropyl]phosphinyl]oxy]-1-oxopropyl]-L-proline, sodium salt, lactose and Avicel and then blending with the corn starch. Magnesium stearate is added and the dry mixture is compressed in a tablet press to form 1000 tablets each containing 50 mg of active ingredient. The tablets are coated with a solution of Methocel E 15 (methyl cellulose) including as a color a lake containing yellow #6.

In a similar manner, tablets containing 50 mg of the product of any of Examples 1 and 3 to 130 can be prepared.

EXAMPLE 134

Two piece #1 gelatin capsules each containing 100 mg of 1-[(S)-2-[[[(±)-1-(benzoylamino)-3-phenylpropyl]phosphinyl]oxy]-1-oxopropyl]-L-proline, sodium salt are filled with a mixture of the following ingredients:

    ______________________________________                                         1-[(S)--2-[[[(±)-1-(Benzoylamino)-3-                                                                 100    mg                                             phenylpropyl]phosphinyl]oxy]-1-                                                oxopropyl]-L-proline, sodium salt                                              Magnesium stearate       7      mg                                             Lactose                  193    mg                                                                      300    mg                                             ______________________________________                                    

In a similar manner, capsules containing 100 mg of the product of any of Examples 1 and 3 to 130 can be prepared.

EXAMPLE 135

An injectable solution is prepared as follows:

    ______________________________________                                         (±)-1-[2-[[Hydroxy(4-phenylbutyl)-                                                                   500    g                                              phosphinyl]oxy]-1-oxopropyl]-                                                  L-proline, disodium salt                                                       Methyl paraben           5      g                                              Propyl paraben           1      g                                              Sodium chloride          25     g                                              Water for injection      5      l                                              ______________________________________                                    

The active substance, preservatives, and sodium chloride are dissolved in 3 liters of water for injection and then the volume is brought up to 5 liters. The solution is filtered through a sterile filter and asceptically filled into presterilized vials which are closed with presterilized rubber closures. Each vial contains 5 ml of solution in a concentration of 100 mg of active ingredient per ml of solution for injection.

In a similar manner, an injectable solution containing 100 mg of active ingredient per ml of solution can be prepared for the product of any of Examples 2 to 130.

EXAMPLE 136

1000 Tablets each containing the following ingredients:

    ______________________________________                                         (±)-1-[2-[[Hydroxy(4-phenylbutyl)-                                                                   100    mg                                             phosphinyl]oxy]-1-oxopropyl]-                                                  L-proline, disodium salt                                                       Avicel                   100    mg                                             Hydrochlorothiazide      12.5   mg                                             Lactose                  113    mg                                             Corn starch              17.5   mg                                             Stearic acid             7      mg                                                                      350    mg                                             ______________________________________                                    

are prepared from sufficient bulk quantities by slugging the (±)-1-[2-[[hydroxy(4-phenylbutyl)phosphinyl]oxy]-1-oxopropyl]-L-proline, disodium salt, Avicel and a portion of the stearic acid. The slugs are ground and passed through a #2 screen, then mixed with the hydrochlorothiazide, lactose, corn starch, and remainder of the stearic acid. The mixture is compressed into 350 mg capsule shaped tablets in a tablet press. The tablets are scored for dividing in half.

In a similar manner, tablets can be prepared containing 100 mg of the product of any of Examples 2 to 130. 

What is claimed is:
 1. A compound of the formula ##STR431## and pharmaceutically acceptable salts thereof wherein: ##STR432## R₄ is hydrogen, lower alkyl, halogen, keto, hydroxy, ##STR433## azido, amino, ##STR434## a 1- or 2-naphthyl of the formula ##STR435## --(CH₂)_(m) --cycloalkyl, ##STR436## --O--lower alkyl, ##STR437## a 1- or 2-naphthyloxy of the formula ##STR438## --S--lower alkyl, ##STR439## or a 1- or 2-naphthylthio of the formula ##STR440## R₅ is keto, halogen, ##STR441## --O--lower alkyl, a 1- or 2-naphthyloxy of the formula ##STR442## --S--lower alkyl, ##STR443## or a 1- or 2-naphthylthio of the formula ##STR444## R₇ is keto or ##STR445## each R₈ is the same or different and is halogen or --Y--R₁₄ ; R₁₁ is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenylmethyl;R₁₂ is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, or hydroxy; m is zero, one, two or three; p is one, two or three provided that p is more than one only if R₁₁ or R₁₂ is hydrogen, methyl, methoxy, chloro, or fluoro; R₁₃ is hydrogen or lower alkyl of 1 to 4 carbons; Y is oxygen or sulfur; R₁₄ is lower alkyl of 1 to 4 carbons, ##STR446## or the R₁₄ groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons or a di(lower alkyl of 1 to 4 carbons) substituent; R₁ is alkyl of 1 to 10 carbons, aminoalkyl, haloalkyl, ##STR447## wherein q is zero or an integer from 1 to 7 and R₁₁ and p are as defined above; and R₁₉ and R₂₀ are independently selected from the group consisting of hydrogen, lower alkyl, halo substituted lower alkyl, ##STR448## wherein m, R₁₁ and p are as defined above; R₂ is hydrogen, lower alkyl, halo substituted lower alkyl, ##STR449## wherein r is as defined hereinbefore; R₃ and R₆ are independently selected from hydrogen, lower alkyl, benzyl, benzhydryl, alkali metal, or ##STR450## wherein R₁₅ is hydrogen, lower alkyl, cycloalkyl, or phenyl, and R₁₆ is hydrogen, lower alkyl, lower alkoxy, phenyl, or R₁₅ and R₁₆ taken together are --(CH₂)₂ --, --(CH₂)₃, --CH═CH-- or ##STR451## R₁₇ is lower alkyl, benzyl, or phenethyl; and R₁₈ is hydrogen, lower alkyl, benzyl, or phenethyl.
 2. A compound of claim 1 wherein:R₄ is hydrogen, hydroxy, chloro, fluoro, lower alkyl of 1 to 4 carbons, cyclohexyl, amino, --O--lower alkyl wherein lower alkyl is of 1 to 4 carbons, ##STR452## 1- or 2-naphthyloxy, --S--lower alkyl wherein lower alkyl is of 1 to 4 carbons, ##STR453## or 1- or 2-naphthylthio; R₅ is --O--lower alkyl wherein lower alkyl is of 1 to 4 carbons, --S--lower alkyl wherein lower alkyl is of 1 to 4 carbons, ##STR454## R₇ is phenyl, 2-hydroxyphenyl, or 4-hydroxyphenyl; each R₈ is fluoro, chloro or --Y--R₁₄ ; Y is oxygen or sulfur; R₁₄ is lower alkyl of 1 to 4 carbons or the R₁₄ groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a methyl or dimethyl substituent; m is zero, one or two; R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy; R₂ is hydrogen, lower alkyl of 1 to 4 carbons, CF₃, or amino substituted lower alkyl of 1 to 4 carbons; R₁ is alkyl of 1 to 10 carbons, ##STR455## --(CH₂)_(q) --cycloalkyl wherein cycloalkyl is of 5 or 6 carbons, ##STR456## wherein q is zero or an integer from 1 to 4 and R₁₁ is as defined above; R₁₉ and R₂₀ are independently selected from lower alkyl of 1 to 4 carbons or ##STR457## wherein q and R₁₁ are as defined above; R₃ and R₆ are independently selected from hydrogen, alkali metal salt, lower alkyl of 1 to 4 carbons, or ##STR458## R₁₅ is hydrogen, methyl or isopropyl; and R₁₆ is lower alkyl of 1 to 4 carbons or phenyl.
 3. A compound of claim 2 wherein X is ##STR459## R₄ is hydrogen, cyclohexyl, lower alkoxy of 1 to 4 carbons, ##STR460## m is zero, one or two; R₁₁ is hydrogen, methyl, methoxy, methylthio, bromo, fluoro, or hydroxy;Y is oxygen or sulfur; t is two or three; and R₆ is hydrogen, ##STR461## or an alkali metal salt, R₁ is ##STR462## R₂ is hydrogen or lower alkyl; and R₃ is hydrogen, alkali metal or phenylalkyl.
 4. A compound of claim 3 whereinR₁ is phenylalkyl or ##STR463## wherein R₁₉ is phenylalkyl and R₂₀ is phenyl; R₂ is hydrogen, methyl, or --(CH₂)₄ --NH₂ ; and R₃ is hydrogen, phenylmethyl, ##STR464## or an alkali metal salt.
 5. A compound of claim 4 wherein R₁ is alkyl of 1 to 10 carbons.
 6. A compound of claim 5 wherein X is ##STR465## and R₄ is hydrogen.
 7. The compound of claim 3 wherein R₁ is phenylbutyl; R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 8. The compound of claim 3 wherein R₁ is ##STR466## R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 9. The compound of claim 6 wherein R₁ is ##STR467## R₂ is methyl; R₃ is ethyl; and R₆ is an alkali metal salt.
 10. The compound of claim 6 wherein R₁ is ##STR468## R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 11. The compound of claim 6 wherein R₁ is ##STR469## R₂ is methyl; R₃ is ##STR470## and R₆ is an alkali metal salt.
 12. The compound of claim 6 wherein R₁ is ##STR471## R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 13. The compound of claim 6 wherein R₁ is ##STR472## R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 14. The compound of claim 6 wherein R₁ is ##STR473## R₂ is methyl; and R₃ and R₆ are an alkali metal salt.
 15. The compound of claim 6 wherein R₁ is ##STR474## R₂ is hydrogen; and R₃ and R₆ are an alkali metal salt.
 16. The compound of claim 6 wherein R₁ is ##STR475## R₂ is hydrogen; and R₃ and R₆ are an alkali metal salt.
 17. The compound of claim 1 wherein X is ##STR476##
 18. The compound of claim 1 wherein R₁ is ##STR477## and R₁₉ and R₂₀ are independently selected from lower alkyl of 1 to 4 carbons or ##STR478## wherein q is zero or an integer from 1 to 4 and R₁₁ is hydrogen, methyl, methoxy, methylthio, chloro, bromo, fluoro, or hydroxy.
 19. A compound of claim 18 wherein X is ##STR479## and R₄ is hydrogen.
 20. A composition useful for treating hypertension comprising a pharmaceutically acceptable carrier and a hypotensive compound or pharmaceutically acceptable salt thereof defined in claim
 1. 21. The method of alleviating hypertension in a mammalian specie which comprises administering an effective amount of the composition of claim
 20. 